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G1777
Sigma
Glial Cell Line-derived Neurotrophic Factor human
>98% (SDS-PAGE and HPLC), recombinant, expressed in Escherichia coli, lyophilized powder, cell culture tested
| Synonym: | GDNF |
| MDL number: | MFCD00285867 |
Description
| Analysis Note | The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures. |
| Physical form | Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin. |
| Biochem/physiol Actions | Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson's disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | >98% (SDS-PAGE and HPLC) |
| form | lyophilized powder |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| Gene Information | human ... GDNF(2668) |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Ibanez, C., Emerging themes in structural biology of neurotrophic factors. Trends Neurosci. 21, 438-444, (1998) |
| Saarma, M., Arumae, U., GDNF family; new promising neurotrophic factors. Neurosci. News 2, 26-34, (1999) | |
| Lin L.-F., et al., GDNF: A glia cell line-derived neurotrophic factor for midbrain dopaminergic neurons. Science 260, 1130-1132, (1993) | |
| Yan, Q., et al., In vivo neurotrophic effects of GDNF on neonatal and adult facial motor neurons,. Nature 373, 341-344, (1995) |
